BACTERIOSTATIC EFFECT OF DIFFERENT AUTOLOGOUS PLATELET CONCENTRATES AGAINST FUSOBACTERIUM NUCLEATUM

Abstract

Background: The use of autologous platelet concentrates (PCs) has gained popularity in a variety of medical fields in the past two decades. Various platelet concentrates have been developed with different centrifugation protocols. The regenerative potential of PCs have been studied and used in variety of periodontal surgical procedure. But there has been only few reports regarding their antimicrobial potential. In this study, we evaluated the antimicrobial effects of PRP, PRF and i-PRF against Fusobacterium nucleatum.

Methods: The aim of this in vitro study was to assess the bacteriostatic effect of different platelet concentrates on the growth of Fusobacterium nucleatum. All groups of metronidazole (positive control), PRP, i-PRF and PRF (the 3 experimental groups) consisted of twenty (20) samples each. All the samples were subjected to a disk diffusion test and incubated for forty eight (48) hours. The bacteriostatic effect was assessed by measuring the zones of inhibition. The results were subjected to statistical analysis using the Shapiro-Wilks test, One way ANOVA, Independent t-test and One sample t-test at a level of significance of p<0.05.

Results: The results of this study demonstrated that PRP and i-PRF are capable of inhibiting F.nucleatum growth at 48 hours and categorized as partially active while majority of the samples with platelet-rich fibrin (PRF) displayed either contact inhibition or very minimal inhibition and categorized as inactive.

Conclusions: In the present study, platelet concentrates were able to demonstrate bacteriostatic activity against F.nucleatum. These PCs could be used as an adjunct in minimizing bacterial contamination.